ving the needle protector. 7. appropriately secure donor tubing to donor's arm. 8. mix blood with anticoagulant at several intervals during collection. 9. collect labeled volume of blood (+/–10%). 10. tighten knot firmly after collection. clamp between knot and "y". sever donor tubing between the knot and clamp. alternate methods may be used to seal tubing. caution do not use the dielectric tube sealer while the needle is connected to the donor's body. anytime before step #13 below, sever donor tubing between the two seals. collect blood samples as follows: a) connect venoject ii multi-sample luer adapter to venoject ii tube holder (fig. 1). b) remove covers and connect multi-sample luer adapter to female luer at end of donor sampling tubing (fig. 2). c) snap cliktip in donor sampling tubing to open blood pathway. insert fig. 1 here insert fig. 2 here d) insert blood collection tube (venoject ii or equivalent) firmly into venoject ii tube holder : when full, remove sample tube from holder. repeat to collect additional samples. 11. reapply clamp to donor tubing between phlebotomy needle and "y"; release pressure on donor's arm and remove needle. caution discard tubing/phlebotomy needle unit according to institutional procedures. 12. immediately after collection, invert bag several times to assure blood and anticoagulant are well mixed. 13. strip blood from the donor tubing into bag, mix well, and allow tubing to refill. seal on or near x marks on donor tubing to provide numbered aliquots of anticoagulated blood for testing. 14. the time of addition of optisol solution may vary depending on the processing option selected. add the solution under one of the following conditions. a) after removal of plasma from freshly collected blood. b) within 8 hours of blood collection if components are prepared. c) within 72 hours of collection if blood is refrigerated immediately following collection. 15. centrifuge the unit to separate red cells from plasma. 16. snap cllktip (inline closure device) of primary collection bag and transfer plasma into satellite bag. clamp transfer tubing of satellite bag. 17. snap cllktip of optlsol solution bag and drain contents into primary bag containing red blood cells. seal tubing of primary bag in two places, and cut between seals and separate from satellite bag(s). note: for teruflex double bags, seal optisol solution bag tubing in two places and cut between seals. discard optisol solution container. 18. invert the red cell- optisol mixture several times to insure the final as-5 red cell product is well suspended. 19. store as-5 red blood cells between 1-6°c. 20. infuse as-5 red blood cells within 42 days of collection. for further processing, use standard component processing techniques. to open blister package, peel cover film back four fifths of its length. after opening, unused bags may be stored for 30 days by returning cover film to original position and sealing with tape to prevent possible loss of moisture. insert fig. 3 here cautions •the packet of ageless contained in this package absorbs oxygen and generates heat on removal and should be handled with care. • dispose with packet in tray. • do not dispose with wastes containing volatile or flammable materials. • discard ageless packet without opening. terumo ® terumo corporation 44-1, 2-chome, hatagaya, shibuya-ku, tokyo, japan ®: registered trademark n-bb-op-a(sp) 3 image fig. 1 (attach luer adapter to tube holder) image figure sealing with tape image fig. 2 (attach adapter/holder to blood sampling arm)